| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.038474 |
| Chromosome: | chromosome_17 |
| Location: | 3204229 |
| Confidence (%): | 95 |
| Locus systemic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g722150 | PKS3 | Type III polyketide synthase | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CATACCCTACTCATTCCCCACAACAGCATC |
| Internal bar code: | TTCTCGTGTTGATAACAGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 964 |
| LEAP-Seq percent confirming: | 99.8192 |
| LEAP-Seq n confirming: | 1104 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCCAAGTAGCCCTCACCT |
| Suggested primer 2: | TGGTCACGTTGATCTTCAGC |