| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.038480 |
| Chromosome: | chromosome 16 |
| Location: | 3554816 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679876 | (1 of 1) PTHR15859//PTHR15859:SF4 - SETA BINDING PROTEIN 1 // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCTGGGCTGAACCGCAGCCGCAGGGTCT |
| Internal bar code: | GTCAGAGGCCGGAAATTCCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 403 |
| LEAP-Seq percent confirming: | 99.6377 |
| LEAP-Seq n confirming: | 3850 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCTCGTTTCTCTGTACCA |
| Suggested primer 2: | CCCCATACGTCTCTCTGAGC |