| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.038492 |
| Chromosome: | chromosome 17 |
| Location: | 4284515 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g730750 | (1 of 1) PTHR10913:SF47 - FOLLISTATIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAGCAGGCAGCCCTCATGCCACGTGTGG |
| Internal bar code: | AATTAAAGTGTCATTCAGACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 976 |
| LEAP-Seq percent confirming: | 67.5236 |
| LEAP-Seq n confirming: | 4439 |
| LEAP-Seq n nonconfirming: | 2135 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCGAACACTCACGGGTTT |
| Suggested primer 2: | GCGGGTCAGTCTTTTAGCAG |