| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.038500 |
| Chromosome: | chromosome_3 |
| Location: | 3777228 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g170100 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGACGAGGACTAAGATGAATGTGTGAACTG |
| Internal bar code: | CGACGAAACCCAGATTTCACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 666 |
| LEAP-Seq percent confirming: | 98.8215 |
| LEAP-Seq n confirming: | 2264 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGCAGGACATTAGCATCT |
| Suggested primer 2: | TGTTGCACCTTTCTTCATGC |