| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.038527 |
| Chromosome: | chromosome 2 |
| Location: | 1862034 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g087450 | (1 of 4) PTHR16255:SF4 - SPORULATION PROTEIN RMD8 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCATTGGGCCGGTGAAGGGCGTTCCGTAG |
| Internal bar code: | TCTTCTGCGTGCGGCATCCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 762 |
| LEAP-Seq percent confirming: | 97.6307 |
| LEAP-Seq n confirming: | 2390 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATCTCACAGGTGGTGGTG |
| Suggested primer 2: | GCATGCCAAGATAGTGCAGA |