Insertion junction: LMJ.RY0402.038527_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.038527
Chromosome:chromosome_2
Location:1862034
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre02.g087450 sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):TTCATTGGGCCGGTGAAGGGCGTTCCGTAG
Internal bar code:TCTTCTGCGTGCGGCATCCAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:762
LEAP-Seq percent confirming:97.6307
LEAP-Seq n confirming:2390
LEAP-Seq n nonconfirming:58
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CAATCTCACAGGTGGTGGTG
Suggested primer 2:GCATGCCAAGATAGTGCAGA