Insertion junction: LMJ.RY0402.038528_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.038528
Chromosome:chromosome_6
Location:6465627
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
None

Insertion site details

Flanking sequence (orientation from cassette outwards):GCTTCGACGGCGCGCGTTACCGGCGCGTCG
Internal bar code:ACCGGCTCGCCTTGTTGATGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:814
LEAP-Seq percent confirming:98.237
LEAP-Seq n confirming:4625
LEAP-Seq n nonconfirming:83
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:TCAGGACGTGACTCTGATGC
Suggested primer 2:GACACTGTGAGGAAGTGGCA