Insertion junction: LMJ.RY0402.038528_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.038528
Chromosome:chromosome 17
Location:2705829
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g718000 PHC7,PHC6 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 7 intron

Insertion site details

Flanking sequence (orientation from cassette outwards):CCCATAGATACTGCCTTGACACCCACGTGA
Internal bar code:CTCTGCGCGTCTCAAAATACAT

Confirmation - LEAP-Seq

LEAP-Seq distance:315
LEAP-Seq percent confirming:99.0459
LEAP-Seq n confirming:9135
LEAP-Seq n nonconfirming:88
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTCTCCTCGTGACCCAAC
Suggested primer 2:CCACAACATCCACTCACCAG