| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.038528 |
| Chromosome: | chromosome_17 |
| Location: | 2705829 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g718000 | PHC7 | Cell wall protein pherophorin-C7 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCCATAGATACTGCCTTGACACCCACGTGA |
| Internal bar code: | CTCTGCGCGTCTCAAAATACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 315 |
| LEAP-Seq percent confirming: | 99.0459 |
| LEAP-Seq n confirming: | 9135 |
| LEAP-Seq n nonconfirming: | 88 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTCTCCTCGTGACCCAAC |
| Suggested primer 2: | CCACAACATCCACTCACCAG |