| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.038528 |
| Chromosome: | chromosome_17 |
| Location: | 2706381 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g718000 | PHC7 | Cell wall protein pherophorin-C7 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGCAGCAAACGGCGTAGCCAGCTCGGCCGT |
| Internal bar code: | GTTACCCTAGGGCGCGTCCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 414 |
| LEAP-Seq percent confirming: | 95.9225 |
| LEAP-Seq n confirming: | 60623 |
| LEAP-Seq n nonconfirming: | 2577 |
| LEAP-Seq n unique pos: | 94 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACACCTGATAGCACCCGTC |
| Suggested primer 2: | ATGCAATGCGATTTGTGTGT |