Insertion junction: LMJ.RY0402.038528_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.038528
Chromosome:chromosome 17
Location:2706381
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g718000 PHC7,PHC6 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 7 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCAGCAAACGGCGTAGCCAGCTCGGCCGT
Internal bar code:GTTACCCTAGGGCGCGTCCCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:414
LEAP-Seq percent confirming:95.9225
LEAP-Seq n confirming:60623
LEAP-Seq n nonconfirming:2577
LEAP-Seq n unique pos:94

Suggested primers for confirmation by PCR

Suggested primer 1:AACACCTGATAGCACCCGTC
Suggested primer 2:ATGCAATGCGATTTGTGTGT