Insertion junction: LMJ.RY0402.038546_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.038546
Chromosome:chromosome_9
Location:7035629
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre09.g411200 TEF5 Rieske [2Fe-2S] domain containing protein antisense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):GGTTGCGAAGTCGTCGCAGGCGCGCGTGCT
Internal bar code:TTAACGTTGCCCCATGTCAAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:849
LEAP-Seq percent confirming:99.7194
LEAP-Seq n confirming:17772
LEAP-Seq n nonconfirming:50
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CTCGATGGCGTAAATCTGGT
Suggested primer 2:ATGCACAAATGCGGATGTAA