| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.038546 |
| Chromosome: | chromosome_9 |
| Location: | 7035629 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g411200 | TEF5 | Rieske [2Fe-2S] domain containing protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGTTGCGAAGTCGTCGCAGGCGCGCGTGCT |
| Internal bar code: | TTAACGTTGCCCCATGTCAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 849 |
| LEAP-Seq percent confirming: | 99.7194 |
| LEAP-Seq n confirming: | 17772 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGATGGCGTAAATCTGGT |
| Suggested primer 2: | ATGCACAAATGCGGATGTAA |