Insertion junction: LMJ.RY0402.038554_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.038554
Chromosome:chromosome_8
Location:2522525
Confidence (%):58
Locus systematic id Locus common name Defline Orientation Feature
None

Insertion site details

Flanking sequence (orientation from cassette outwards):ATCAGACCGCATGAGACCATGTGTGTGTTG
Internal bar code:GGACAGTTTGAGGTCATGCCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:83
LEAP-Seq percent confirming:11.1111
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AAAACCCTGAGGCCATTCTT
Suggested primer 2:GGCTTTTATACCGGATGGGT