| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.038577 |
| Chromosome: | chromosome_3 |
| Location: | 8176241 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g200351 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGCCAATGAGGCGTCGCTGGCGGCGGTGG |
| Internal bar code: | GGGCCCTTACGGGATGAGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 362 |
| LEAP-Seq percent confirming: | 97.5105 |
| LEAP-Seq n confirming: | 3486 |
| LEAP-Seq n nonconfirming: | 89 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGGTTGTGATGTGGTTGC |
| Suggested primer 2: | CAATGTCGGCGAAGAGTGTA |