| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.038581 |
| Chromosome: | chromosome_6 |
| Location: | 881645 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g254917 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TAGTGCAGCTGGGCACTGAATGAGAGCCGA |
| Internal bar code: | CGCCCGCATCCAGCGCCAAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 461 |
| LEAP-Seq percent confirming: | 99.7305 |
| LEAP-Seq n confirming: | 3331 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGGAGTGAAGAGCCGAAA |
| Suggested primer 2: | AACGTTATTGCCAAACCTGG |