Insertion junction: LMJ.RY0402.038586_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.038586
Chromosome:chromosome_8
Location:4265813
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre08.g380950 sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):TGTTCCTAATCTACCTAAATTTGTTGGGAG
Internal bar code:GTACCTCGCTTGCGGGGTGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:816
LEAP-Seq percent confirming:98.5402
LEAP-Seq n confirming:14581
LEAP-Seq n nonconfirming:216
LEAP-Seq n unique pos:90

Suggested primers for confirmation by PCR

Suggested primer 1:ACCAATGTGGTGGTGGATGT
Suggested primer 2:ACATTTCAGTTTGTGCAGCG