Insertion junction: LMJ.RY0402.038600_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.038600
Chromosome:chromosome_9
Location:4986335
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre09.g397993 Similar to Flagellar Associated Protein FAP201 sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TGGGGGGTGCGGAGGGGTTAGAAGCGATTG
Internal bar code:AGTCGTCTGATCCAAGGGGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:246
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:AAAGGGAAGGGACATTGCTT
Suggested primer 2:TGGTTCTGGGTCTTGGTTTC