| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.038607 |
| Chromosome: | chromosome 2 |
| Location: | 3911335 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g096300 | CIA5,CCM1 | Regulator of CO2-responsive genes | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATCCGGGCCTTGGGTGCCGGAGCTGGTG |
| Internal bar code: | CTCGGTAGTGCACGGTGGAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 447 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTAATGTACGACCACACGG |
| Suggested primer 2: | ACACACCAGACACACACGGT |