Insertion junction: LMJ.RY0402.038614_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.038614
Chromosome:chromosome_15
Location:987221
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre15.g639050 antisense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):CGGCAGCCCCGCTCCAGCACCCGCAGCAGC
Internal bar code:TTCCCCAGAGTGAACATACGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:491
LEAP-Seq percent confirming:77.829
LEAP-Seq n confirming:3377
LEAP-Seq n nonconfirming:962
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GAACGTCCTCTAACGCCTTG
Suggested primer 2:CACCGTTTGCAAAGGAAGAT