| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.038614 |
| Chromosome: | scaffold_43 |
| Location: | 1868 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre43.g760497 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGCCACCCCCTGCCCCTGCCGCGCAGGCCA |
| Internal bar code: | GTGATGGGACCATCGGACCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 161 |
| LEAP-Seq percent confirming: | 29.0989 |
| LEAP-Seq n confirming: | 859 |
| LEAP-Seq n nonconfirming: | 2093 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTGTGTGTGGAGGTGTG |
| Suggested primer 2: | TGGTGAAGAGCATGTTCGAG |