Insertion junction: LMJ.RY0402.038621_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.038621
Chromosome:chromosome_9
Location:5603006
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre09.g400886 sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):GGGATCTTTACATGCTCCTTTCAGCAGCGG
Internal bar code:ACACGCCCCCGGTCGAACTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:410
LEAP-Seq percent confirming:40.1803
LEAP-Seq n confirming:1248
LEAP-Seq n nonconfirming:1858
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:TGACAGCCTCTAACAGCACG
Suggested primer 2:TTCGTACAATGCCTGTGAGC