Insertion junction: LMJ.RY0402.038621_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.038621
Chromosome:chromosome_9
Location:5603007
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre09.g400886 sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):CGATGGCCGCTCCGCACCTTGCATCCACCG
Internal bar code:ACCAGGGTCCTGCGTGTCTATG

Confirmation - LEAP-Seq

LEAP-Seq distance:129
LEAP-Seq percent confirming:87.8049
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGACAGCCTCTAACAGCACG
Suggested primer 2:TTCGTACAATGCCTGTGAGC