| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.038634 |
| Chromosome: | chromosome 6 |
| Location: | 1826916 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g262750 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTCGGTAGCCTGGCCAGCGCCGGGGTTC |
| Internal bar code: | GTACGCGGCGAGCGTTGGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 138 |
| LEAP-Seq percent confirming: | 93.7103 |
| LEAP-Seq n confirming: | 1326 |
| LEAP-Seq n nonconfirming: | 89 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAAAGCTTGTTGCGATGA |
| Suggested primer 2: | CCCTGTGTACCGCAACTAGG |