| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.038649 |
| Chromosome: | chromosome_2 |
| Location: | 5173600 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g105500 | AOD1 | Acetylornithine deacetylase | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGTCCATCACCTGGAGGGGGTTACATTCA |
| Internal bar code: | AGTTTTTCCTTTCAGTAATAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1106 |
| LEAP-Seq percent confirming: | 98.9833 |
| LEAP-Seq n confirming: | 3310 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAGGCAGACACCTGTGAG |
| Suggested primer 2: | TCCATCCTATGCTGCTACCC |