| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.038651 |
| Chromosome: | chromosome_14 |
| Location: | 2814406 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g627411 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATCCGCCACTGCCGGGGCCAATGCCTCTAA |
| Internal bar code: | GCTTACCATCCACCGGGCGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 775 |
| LEAP-Seq percent confirming: | 93.8654 |
| LEAP-Seq n confirming: | 12256 |
| LEAP-Seq n nonconfirming: | 801 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGTGGTCATGCATGGTAG |
| Suggested primer 2: | GAGAGTGACAGCGACAGCAC |