Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.038654 |
Chromosome: | chromosome_16 |
Location: | 5067943 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g684250 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACCTCCGAGCCGCGTCTCATGCTCCAAATC |
Internal bar code: | TGGAAACGGGAGGAGTGTCAGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 613 |
LEAP-Seq percent confirming: | 99.7038 |
LEAP-Seq n confirming: | 1683 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGTCAATCCCAAGCTGTTGT |
Suggested primer 2: | CGCATTCTCACCATGTCATC |