| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.038678 |
| Chromosome: | chromosome 2 |
| Location: | 5379485 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g107300 | DHDPS,DPS1 | (1 of 1) 4.3.3.7 - 4-hydroxy-tetrahydrodipicolinate synthase / Dihydrodipicolinate synthetase; Dihydrodipicolinate synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTAGCGACGTGCGTGTAGAAGGCTCTTG |
| Internal bar code: | ACGGAGTCCAAAGCGCCTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 822 |
| LEAP-Seq percent confirming: | 99.4693 |
| LEAP-Seq n confirming: | 7123 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTAAGAACAGGGGGCGAT |
| Suggested primer 2: | CTAAGGCAGCAATTCAAGGC |