Insertion junction: LMJ.RY0402.038698_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.038698
Chromosome:chromosome_7
Location:1011458
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre07.g319350 sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):GCTGCAGACGAAGCCGCCGCGCCTCTTGTG
Internal bar code:TGCGTCACGGGCAAACGAAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:848
LEAP-Seq percent confirming:85.1934
LEAP-Seq n confirming:3084
LEAP-Seq n nonconfirming:536
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:AAGTAAACGGCCCAGGAAGT
Suggested primer 2:GAGAGGCAGGCATCGAGTAG