Insertion junction: LMJ.RY0402.038698_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.038698
Chromosome:chromosome_7
Location:1011491
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre07.g319350 sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):CGCCAGCGTCAGCCGCTATTGCGGCAGCAA
Internal bar code:CAGTGTCAAAAGCCAGTGAAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:187
LEAP-Seq percent confirming:99.3939
LEAP-Seq n confirming:328
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AAGTAAACGGCCCAGGAAGT
Suggested primer 2:GAGAGGCAGGCATCGAGTAG