| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.038713 |
| Chromosome: | chromosome_16 |
| Location: | 5827931 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g677920 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGAAACCCCCTTCTCTCACACTCCACACAC |
| Internal bar code: | ACGAATAGATGTGCGCTGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 981 |
| LEAP-Seq percent confirming: | 98.797 |
| LEAP-Seq n confirming: | 4517 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACGAGAGGTTTTACGAGC |
| Suggested primer 2: | TGCGTGAAAGAGCAGAGCTA |