| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.038717 |
| Chromosome: | chromosome 1 |
| Location: | 6936632 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g049950 | VLE1,SPO | Sporangin (Vegetative Lytic Enzyme 1); (1 of 14) 3.4.21.62 - Subtilisin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGTATATGTGGCTCTGTACGACATCTCG |
| Internal bar code: | CGAAAATAGCACCTTTCATGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 949 |
| LEAP-Seq percent confirming: | 98.8477 |
| LEAP-Seq n confirming: | 5061 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCCGACTGACTCGCTCTT |
| Suggested primer 2: | TCTTTCGATTTGAAGGGTGG |