Insertion junction: LMJ.RY0402.038719_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.038719
Chromosome:chromosome 7
Location:4486715
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g343400 (1 of 12) IPR000595//IPR018490 - Cyclic nucleotide-binding domain // Cyclic nucleotide-binding-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTGCCCCCACACTTCACACACACGCGCC
Internal bar code:GAGTGCGGATTCGGAACGACAA

Confirmation - LEAP-Seq

LEAP-Seq distance:246
LEAP-Seq percent confirming:99.5985
LEAP-Seq n confirming:2729
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:AGCGTCAAAGAACTCGTCGT
Suggested primer 2:CGGGTTTGGAGAGTGTTTGT