| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.038723 |
| Chromosome: | chromosome_17 |
| Location: | 4114538 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g729550 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCCATGGTAACTGCAACGTCACACACACA |
| Internal bar code: | AAGCGACGATCGCGTCTAATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 677 |
| LEAP-Seq percent confirming: | 90.0884 |
| LEAP-Seq n confirming: | 20887 |
| LEAP-Seq n nonconfirming: | 2298 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTGTTTGCGTACCGTCCT |
| Suggested primer 2: | GAGCACCTTTATCACCGCTC |