| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.038724 |
| Chromosome: | chromosome_12 |
| Location: | 1081074 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g490850 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGCGGCGCAGTACCCCGGACCCATGGGGCC |
| Internal bar code: | CATTGATGTTCTTCAAAAGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 816 |
| LEAP-Seq percent confirming: | 85.5994 |
| LEAP-Seq n confirming: | 1064 |
| LEAP-Seq n nonconfirming: | 179 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGTTCTCACTGCCTCACG |
| Suggested primer 2: | CAACCTCACACCACCTCCTT |