| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.038727 |
| Chromosome: | chromosome 11 |
| Location: | 397511 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467580 | FAP370 | Flagellar Associated Protein 370 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCCTGCAGCCCAGTCGACAGCTCCTCGA |
| Internal bar code: | GGGGGATAGATTTCTCTAAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 282 |
| LEAP-Seq percent confirming: | 97.5207 |
| LEAP-Seq n confirming: | 118 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGATCTCGTGCATGCCTT |
| Suggested primer 2: | TGCTGCTGCTACTTCCAATG |