Insertion junction: LMJ.RY0402.038732_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.038732
Chromosome:chromosome 6
Location:6365951
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g292150 (1 of 2) PF13499//PF13833 - EF-hand domain pair (EF-hand_7) // EF-hand domain pair (EF-hand_8) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGTCCGGTCGCGCTTTGGCCCGTCGCAC
Internal bar code:CAGACGACTTATTGGCAACGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:289
LEAP-Seq percent confirming:97.4255
LEAP-Seq n confirming:2649
LEAP-Seq n nonconfirming:70
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:TGCAGCTAATTCCTGACACG
Suggested primer 2:GCTGTCCAGCTCAAACATGA