Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.038732 |
Chromosome: | chromosome 6 |
Location: | 6365951 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g292150 | (1 of 2) PF13499//PF13833 - EF-hand domain pair (EF-hand_7) // EF-hand domain pair (EF-hand_8) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTCCGGTCGCGCTTTGGCCCGTCGCAC |
Internal bar code: | CAGACGACTTATTGGCAACGAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 289 |
LEAP-Seq percent confirming: | 97.4255 |
LEAP-Seq n confirming: | 2649 |
LEAP-Seq n nonconfirming: | 70 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCAGCTAATTCCTGACACG |
Suggested primer 2: | GCTGTCCAGCTCAAACATGA |