| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.038738 |
| Chromosome: | chromosome_13 |
| Location: | 2804698 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g583000 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCAGACCTGCAGCCAGTGTGTGTAAGGGCG |
| Internal bar code: | TTGCATTGCGGTGCCGGCTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1131 |
| LEAP-Seq percent confirming: | 99.3689 |
| LEAP-Seq n confirming: | 18581 |
| LEAP-Seq n nonconfirming: | 118 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCACCGAGCTGAATACTG |
| Suggested primer 2: | GCATTACCGATGGTCGTCTT |