| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.038754 |
| Chromosome: | chromosome_2 |
| Location: | 4868405 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g103250 | MRP5 | ABC transporter, multidrug resistance associated protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCGTGTCCAACATCGCCGTACGGGCCACAC |
| Internal bar code: | AGCACAATCTGGTGGCCGCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 440 |
| LEAP-Seq percent confirming: | 97.3854 |
| LEAP-Seq n confirming: | 7077 |
| LEAP-Seq n nonconfirming: | 190 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTGACAAGGACCAAACAC |
| Suggested primer 2: | CGTTCGCATGAATGTGACTC |