Insertion junction: LMJ.RY0402.038766_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.038766
Chromosome:chromosome 2
Location:1747028
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g086400 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGTGATGCACACCCTAGGCGCCTTGCCAA
Internal bar code:ACAGGACTCCGGCTCACCACCG

Confirmation - LEAP-Seq

LEAP-Seq distance:738
LEAP-Seq percent confirming:99.542
LEAP-Seq n confirming:8477
LEAP-Seq n nonconfirming:39
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:AAAATTTTTCTCCGCCTTCG
Suggested primer 2:GCGTGACCTTCCTTCTTCAG