Insertion junction: LMJ.RY0402.038772_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.038772
Chromosome:chromosome 6
Location:7222564
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g298000 (1 of 24) IPR009091 - Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTAGCTCGGGCAGGTGCCGCGGCGCTGG
Internal bar code:TTGGGTCTGCCGGGCCCGACCC

Confirmation - LEAP-Seq

LEAP-Seq distance:404
LEAP-Seq percent confirming:99.8472
LEAP-Seq n confirming:5226
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AAAATGGATGGGAGGGTTTC
Suggested primer 2:CCGACTCTCCATGTCAGGTT