| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.038773 |
| Chromosome: | chromosome_10 |
| Location: | 746722 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g422800 | HER1 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AACGTACACCAGCGTCCCAAAGTCTTCAAA |
| Internal bar code: | GGACAAACCCGGTCTGTCGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 725 |
| LEAP-Seq percent confirming: | 98.4061 |
| LEAP-Seq n confirming: | 1667 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGTCCCGCATTGATAAAA |
| Suggested primer 2: | ACCATAATGCCATCCGTCAT |