| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.038773 |
| Chromosome: | chromosome 10 |
| Location: | 746722 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422800 | HER1 | DnaJ-domain protein; (1 of 1) PF00226//PF00570 - DnaJ domain (DnaJ) // HRDC domain (HRDC) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGGTCACAGGCGTGGGCGGTTAGGCCC |
| Internal bar code: | AGCCCTTGCTGACTGTCTGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1139 |
| LEAP-Seq percent confirming: | 99.7675 |
| LEAP-Seq n confirming: | 2575 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGTCCCGCATTGATAAAA |
| Suggested primer 2: | ACCATAATGCCATCCGTCAT |