| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.038777 |
| Chromosome: | chromosome_7 |
| Location: | 1117355 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g320200 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTTGGGGGTGATTGATGCACACACGTGGAG |
| Internal bar code: | CTCGGGCTGGACGATCTGCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 541 |
| LEAP-Seq percent confirming: | 98.0299 |
| LEAP-Seq n confirming: | 3732 |
| LEAP-Seq n nonconfirming: | 75 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTCTAGAAGACCAGCCAC |
| Suggested primer 2: | ACGTTGCTGCTGTCACAAAG |