| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.038794 |
| Chromosome: | chromosome_2 |
| Location: | 6754962 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g118500 | COPB2 | Beta\' subunit of COP-I complex | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGAGGGCGGCCTGGAGGGGCTGCTGGCGC |
| Internal bar code: | GTGTGGTGATGACATCCACGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 215 |
| LEAP-Seq percent confirming: | 67.1329 |
| LEAP-Seq n confirming: | 192 |
| LEAP-Seq n nonconfirming: | 94 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAATGGGCCTTTGCTCCTT |
| Suggested primer 2: | CTCCTCTTCCTCCTCAGGCT |