| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.038794 |
| Chromosome: | chromosome 2 |
| Location: | 6754962 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g118500 | COPB2 | (1 of 1) K17302 - coatomer, subunit beta' (COPB2, SEC27); Beta' subunit of COP-I complex | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGGACGCCGCGATGGCGGCCGCCTCCA |
| Internal bar code: | CCGGGCCGAATGGGTGGACACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 703 |
| LEAP-Seq percent confirming: | 99.3735 |
| LEAP-Seq n confirming: | 6345 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAATGGGCCTTTGCTCCTT |
| Suggested primer 2: | CTCCTCTTCCTCCTCAGGCT |