| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.038816 |
| Chromosome: | chromosome_11 |
| Location: | 318609 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g467565 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGGAAATGGGGAGTGCGGAGACAAAAGATC |
| Internal bar code: | ACCTCGGTCGGGAACCAGAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 972 |
| LEAP-Seq percent confirming: | 99.1325 |
| LEAP-Seq n confirming: | 6285 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGAGATACCGTAGTGCCG |
| Suggested primer 2: | CAGTTCCCATTCAACACACG |