Insertion junction: LMJ.RY0402.038822_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.038822
Chromosome:chromosome 7
Location:2208983
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g327150 (1 of 2) IPR001810//IPR003347 - F-box domain // JmjC domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGAGCAGGGCGGTAGGGTGTGGAGTCAGG
Internal bar code:CTTACCTTCGTCACGCGGCCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:476
LEAP-Seq percent confirming:99.7407
LEAP-Seq n confirming:2693
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCACAATTACAACCACAG
Suggested primer 2:TAGCATGCAGCAACAAGACC