| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.038823 |
| Chromosome: | chromosome 6 |
| Location: | 3976818 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278194 | FAP236 | (1 of 3) PF05217 - STOP protein (STOP); Flagellar Associated Protein 236 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTCATGAGGTGTGCGGCGGGGGGCTGG |
| Internal bar code: | CCGTCTCTTGTCAGAGGGCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 797 |
| LEAP-Seq percent confirming: | 99.3648 |
| LEAP-Seq n confirming: | 3911 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTGTGGTTGGCAAAAAT |
| Suggested primer 2: | GCAACAGCTCTCGGATTAGG |