| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.038827 |
| Chromosome: | chromosome 3 |
| Location: | 6154153 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g192000 | SEC13L,SEH1 | COP-II coat subunit, nucleoporin; (1 of 1) K14299 - nucleoporin SEH1 (SEH1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAGCTGGACAGCATGAAACTGAACACCTG |
| Internal bar code: | TTGTTAGGACCGTTGGCCGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 878 |
| LEAP-Seq percent confirming: | 93.7843 |
| LEAP-Seq n confirming: | 3244 |
| LEAP-Seq n nonconfirming: | 215 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGTTGTCCGTGTCTTTAT |
| Suggested primer 2: | AGTCCCCCTCACCTTGACTT |