Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.038835 |
Chromosome: | chromosome_9 |
Location: | 5160294 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g399067 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGGCGGTTTAAGAGAATGAGGACAGACAGC |
Internal bar code: | GCGTCGCCCGTCTTTTGGCTGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 727 |
LEAP-Seq percent confirming: | 96.5116 |
LEAP-Seq n confirming: | 83 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTTTGTTGAGCCACTCCTC |
Suggested primer 2: | GGTCGGTGACCTACTGGCTA |