| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.038836 |
| Chromosome: | chromosome 4 |
| Location: | 2587207 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g222600 | PHC46 | Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACATCATAACACAGCATCTGCTCAGGCCT |
| Internal bar code: | CAATAAGGGTGATGACAAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 332 |
| LEAP-Seq percent confirming: | 97.1643 |
| LEAP-Seq n confirming: | 4009 |
| LEAP-Seq n nonconfirming: | 117 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGCTATGCAGTGACGAG |
| Suggested primer 2: | AGTGACCTCAGCCCTCAAGA |