Insertion junction: LMJ.RY0402.038837_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.038837
Chromosome:chromosome 5
Location:1170441
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g245550 PIK1 (1 of 1) PTHR10048//PTHR10048:SF15 - PHOSPHATIDYLINOSITOL KINASE // PHOSPHATIDYLINOSITOL 4-KINASE ALPHA-LIKE PROTEIN P2-RELATED; Phosphatidylinositol 4-kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAACCAAGACCCAGAACCAGGGATGGGGG
Internal bar code:GGTCGGTCGCATTTTTTCCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:852
LEAP-Seq percent confirming:76.9737
LEAP-Seq n confirming:117
LEAP-Seq n nonconfirming:35
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCGTTTTAGCTCGGTTTG
Suggested primer 2:GCCCAAACTAAACCAAACGA