| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.038837 |
| Chromosome: | chromosome_5 |
| Location: | 1170441 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g245550 | PIK1 | Phosphatidylinositol 4-kinase | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAAACCAAGACCCAGAACCAGGGATGGGGG |
| Internal bar code: | GGTCGGTCGCATTTTTTCCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 852 |
| LEAP-Seq percent confirming: | 76.9737 |
| LEAP-Seq n confirming: | 117 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCGTTTTAGCTCGGTTTG |
| Suggested primer 2: | GCCCAAACTAAACCAAACGA |