| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.038840 |
| Chromosome: | chromosome 4 |
| Location: | 1407792 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g214350 | DIV13,POLA1 | (1 of 1) K02320 - DNA polymerase alpha subunit A (POLA1); DNA polymerase alpha, catalytic subunit | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGTGTGTGGGTGCACTAGCAGGTGCTTG |
| Internal bar code: | ATCCCGCATGTCGGCAGGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 343 |
| LEAP-Seq percent confirming: | 99.4977 |
| LEAP-Seq n confirming: | 2179 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACTCCGTCTTCTTCACGC |
| Suggested primer 2: | CTTGACAGTTGACACCCCCT |